Objective To study the identification of Folium Apocyni Veneti and its adulterant.Methods Folium Apocyni Veneti and Folium Poacyni Hendersonni were identified by traditional methods and modern technology.Results Folium Apocyni Veneti and Folium Poacyni Hendersonni showed that it was difference in materia medica descriptions,microscope characteristic,TLC and HPLC fingerprint graphics.Conclusion The distinct characteristic features revealed in this studies can serve as evidence for the identification of Folium Apocyni Veneti.