Effect of over-expression of GCNF on biological behaviors of HeLa cells

Objective:To transfect cervical cancer HeLa cells with constructed successfully human pcDNA3.1-GCNF eukaryotic expression vector,and observe the effect of GCNF gene on biological behaviors of HeLa cells.Methods:HeLa cells were transfected with recombinant pcDNA3.1-GCNF plasmid;MTT colorimetric method,matrigel adhesion experiment,and Transwell invasive test were used to analyze proliferation,adhesion,and invasion abilities of HeLa cells after transfection.Results:HeLa cells after transient transfection with pcDNA3.1-GCNF plasmid were obtained successfully,the cell proliferation speeds in pcDNA3.1-GCNF plasmid transfection group,null vector transfection group,and non-transfection group were(0.239±0.014),(0.198±0.020),and(0.204±0.012),respectively,the cell proliferation speed in pcDNA3.1-GCNF plasmid transfection group was the fastest(P0.05);after transfection,the adhesion and invasion abilities of HeLa cells didn't change significantly.Conclusion:GCNF gene promotes the proliferation of cervical cancer HeLa cells in vitro,but it has no impact on adhesion and invasion abilities of HeLa cells.