Study on Mutation of High-yielding Strain by Injecting Nitrogen Ion into Validamycin-producing Strain

The application of nitrogen ion beam injection technique in the mutation of high-yielding validamycin-producing strain was explored.After injecting 15×1013 nitrogen ions per metric centimeter with the intensity of 10 keV into validamycin-producing strain for mutation,the regenerated single-colonies were inoculated in shake flasks for the selecting of high-yielding strains.A mutative strain 9275# was obtained,the content of effective component A in strain 9275# was 33.52% higher than that in the original strain,and 9275# had high genetic stability.