Objective:To explore the effect of polymyxin B against multi-resistant pseudomonas aeruginosa(PAE). Methods:23 strains isolated from multi-resistant PAE in the instrument were selected for agar diffusion anti-microbial sensitization test of Polymyxin B. Results:All strains of multi-resistant PAE were sensitive to Polymyxin B. The zone diameter lay in 12~14 mm. Conclusion:Serious infectious patients by multi-resistant PAE were suggested to treat with Polymyxin B in different ways.