Construction of VP2 eukaryotic expression vector of infectious bursal disease virus and its application

The cDNA fragment of protective antigen VP2 gene of infectious bursal disease virus JS strain was amplified by RT-PCR,subcloned into pGEM-T easy vector and then into pcDNA3.1/zeo(+)vector,designated as pcDVP2.By sequencing,the VP2 gene has high homology to that of very virulent IBDV strains.The transiently expression of VP2 in COS-1 cells was detected with the Mab specific to IBDV by the immunflurescent assay.After injection with pcDVP2 DNA to SPF chicken,the results showed that the specific anti-IBDV antibody could be detected in 14 days,67 % of the chickens were protected after challenge with IBDV JS strain.