Objective To observe the influence of eletro-acupuncture on the glutamine(Glu) in the striatum of Parkinson's disease(PD) rats, Methods The rat model of PD was established by injuring the unilateral nigra. The contents of Glu in the bilateral striatum in normal group, sham operation group, electro-acupuncture group and model group were assessed. Results Electro-acupuncture could reduce the content of Glu obviously(P0.05). Conclusion Glu has a close relation with the development of PD, and electro-acupuncture can reduce the release of Glu to treat PD.