Analysis of genotype of 7 variable number tandem repeat of leptospira interrogans in Sichuan

OBJECTIVE To understand the genotype,geography distribution and dominant genotype of leptospirosis in Si Chuan province,in order to provide the scientific evidence for determining the outbreak,prevalent trace,infection source and diabetic foot,and to prevent and control the epidemic.METHODS 7 VNTRs(variable numbers tandem repeats)of 185 leptospirosis of SiChuan were detected by multiple locus variable number of tandem repeats analysis(MLVA),and the results were analyzed by software BioNumerics.RESULTS 164 strains of 185 leptospirosis were successfully amplificated,and the positive rate was 88.6%,which were divided into 81 genotypes.144 of 156 leptospia interrogans of Sichuan were successfully amplificated,and the positive rate was 92.3%,which were divided into 65 genotype,including 11 serogroups and 34 serotypes.CONCLUSION The leptospirosis of Sichuan demonstrates good polymorphism,and genotypes are related to geography distribution.It can provide timely and exactly scientific evidence for molecular epidemiology investigation of leptospira by MLVA method.