清晨好,您是今天最早来到科研通的研友!由于当前在线用户较少,发布求助请尽量完整的填写文献信息,科研通机器人24小时在线,伴您科研之路漫漫前行!

Identification of mitochondria-related hub genes in sarcopenia and functional regulation of MFG-E8 on ROS-mediated mitochondrial dysfunction and cell cycle arrest

肌萎缩 小桶 生物 线粒体 基因 细胞周期 基因表达 细胞生物学 遗传学 转录组 内分泌学
作者
He Li,Kaifang Guan,Dandan Liu,Min Liu
出处
期刊:Food & Function [The Royal Society of Chemistry]
卷期号:13 (2): 624-638 被引量:10
标识
DOI:10.1039/d1fo02610k
摘要

Sarcopenia has high prevalence in the elderly population, but the genes and pathways related to aging in elderly patients with sarcopenia are poorly understood. Milk fat globule epidermal growth factor 8 (MFG-E8) is a peripheral membrane glycoprotein isolated from the milk fat globule membrane (MFGM). It has been found to exhibit various nutritional effects, including antibacterial, anti-cancer, anti-oxidant, anti-sarcopenia, and improving brain development and cognitive effects. This study aimed to investigate key differentially expressed genes (DEGs) and pathways associated with the progression of sarcopenia using bioinformatics analysis and in vitro myoblast experiment. The gene expression profiles of GSE8479 and GSE9676, which includes 40 young normal samples and 55 elderly samples, were downloaded from the Gene Expression Omnibus Database (GEO). Over 3253 DEGs were identified in the young and elderly samples (adjusted p value <0.05). A total of 213 co-expressed significantly DEGs were identified with Venn diagrams, including 82 up-regulated DEGs and 131 down-regulated DEGs. Based on the analysis of Gene Ontology (GO), protein-protein interaction (PPI) networks and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway, 10 hub genes screened by our study have been proved to play a role in regulating the occurrence and development of aging-related sarcopenia mainly via metabolic pathways, Huntington's disease, Parkinson's disease, oxidative phosphorylation and non-alcoholic fatty liver disease pathways. To further verify the protective effect of MFG-E8 on oxidative stress injured myoblasts, the cell cycle distribution, cell viability and reactive oxygen species (ROS) production were measured. The protein and mRNA levels of Akt, extracellular regulated protein kinases (ERK), p21Cip1, p27Kip1, cyclin D1, cyclin E1, cyclin-dependent kinase (CDK) 2 and 4 were quantified using qRT-PCR and western blot analysis. The results indicated that MFG-E8 has potential anti-sarcopenia effects by promoting ERK and Akt activation-mediated cell proliferation and cell cycle progression in myoblasts.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
kehe!完成签到 ,获得积分0
4秒前
霸霸斌完成签到 ,获得积分10
8秒前
gmc完成签到 ,获得积分10
48秒前
谷子完成签到 ,获得积分10
49秒前
数乱了梨花完成签到 ,获得积分10
57秒前
寒战完成签到 ,获得积分10
1分钟前
沐浠完成签到 ,获得积分10
1分钟前
salty完成签到 ,获得积分10
1分钟前
桐桐应助123采纳,获得10
1分钟前
Shandongdaxiu完成签到 ,获得积分10
1分钟前
HCKACECE完成签到 ,获得积分10
1分钟前
Vicky完成签到 ,获得积分10
1分钟前
1分钟前
丰富的绮山完成签到,获得积分10
1分钟前
烟花应助科研通管家采纳,获得30
1分钟前
Akim应助科研通管家采纳,获得30
1分钟前
123发布了新的文献求助10
1分钟前
段采萱完成签到 ,获得积分10
1分钟前
Ava应助123采纳,获得30
2分钟前
leng完成签到 ,获得积分10
2分钟前
dragonhmw完成签到 ,获得积分10
2分钟前
烟花应助依居采纳,获得10
2分钟前
玉一一完成签到 ,获得积分10
2分钟前
哈拉斯完成签到,获得积分10
2分钟前
2分钟前
依居发布了新的文献求助10
2分钟前
蓝胖胖蓝完成签到,获得积分10
2分钟前
3分钟前
稀松完成签到,获得积分10
3分钟前
3分钟前
陌子完成签到 ,获得积分10
3分钟前
zjq完成签到 ,获得积分10
3分钟前
3分钟前
飞云完成签到 ,获得积分10
3分钟前
铁妹儿完成签到 ,获得积分10
3分钟前
SHD完成签到 ,获得积分10
4分钟前
煜琪完成签到 ,获得积分10
4分钟前
高贵逍遥完成签到 ,获得积分10
4分钟前
wyh295352318完成签到 ,获得积分10
4分钟前
jie完成签到 ,获得积分10
4分钟前
高分求助中
좌파는 어떻게 좌파가 됐나:한국 급진노동운동의 형성과 궤적 2500
Sustainability in Tides Chemistry 1500
TM 5-855-1(Fundamentals of protective design for conventional weapons) 1000
Cognitive linguistics critical concepts in linguistics 800
Threaded Harmony: A Sustainable Approach to Fashion 799
Livre et militantisme : La Cité éditeur 1958-1967 500
氟盐冷却高温堆非能动余热排出性能及安全分析研究 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3052644
求助须知:如何正确求助?哪些是违规求助? 2709863
关于积分的说明 7418252
捐赠科研通 2354395
什么是DOI,文献DOI怎么找? 1246007
科研通“疑难数据库(出版商)”最低求助积分说明 605951
版权声明 595921