生物
MAPK/ERK通路
核蛋白
细胞生物学
磷酸化
激酶
病毒学
蛋白激酶A
免疫
信号转导
褐飞虱
普通大麦
细胞外
RNA干扰
免疫系统
病毒
核糖核酸
生物化学
遗传学
植物
禾本科
基因
作者
Zhi-Hang Ding,Qijie Gao,Xin Tong,Wenya Xu,Lulu Ma,Zhen-Jia Zhang,Ying Wang,Xianbing Wang
出处
期刊:The Plant Cell
[Oxford University Press]
日期:2022-05-14
卷期号:34 (8): 3110-3127
被引量:8
标识
DOI:10.1093/plcell/koac143
摘要
Signaling by the evolutionarily conserved mitogen-activated protein kinase or extracellular signal-regulated kinase (MAPK/ERK) plays critical roles in converting extracellular stimuli into immune responses. However, whether MAPK/ERK signaling induces virus immunity by directly phosphorylating viral effectors remains largely unknown. Barley yellow striate mosaic virus (BYSMV) is an economically important plant cytorhabdovirus that is transmitted by the small brown planthopper (SBPH, Laodelphax striatellus) in a propagative manner. Here, we found that the barley (Hordeum vulgare) MAPK MPK3 (HvMPK3) and the planthopper ERK (LsERK) proteins interact with the BYSMV nucleoprotein (N) and directly phosphorylate N protein primarily on serine 290. The overexpression of HvMPK3 inhibited BYSMV infection, whereas barley plants treated with the MAPK pathway inhibitor U0126 displayed greater susceptibility to BYSMV. Moreover, knockdown of LsERK promoted virus infection in SBPHs. A phosphomimetic mutant of the N Ser290 (S290D) completely abolished virus infection because of impaired self-interaction of BYSMV N and formation of unstable N-RNA complexes. Altogether, our results demonstrate that the conserved MAPK and ERK directly phosphorylate the viral nucleoprotein to trigger immunity against cross-kingdom infection of BYSMV in host plants and its insect vectors.
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