炎症
硫化氢
体内
荧光
荧光寿命成像显微镜
生物标志物
病理
材料科学
纳米技术
医学
化学
免疫学
生物
生物化学
光学
硫黄
物理
生物技术
冶金
作者
Donghao Ding,Jiangfeng Li,Lizhen Xu,Jiangyan Wang,Dan Tan,Weiying Lin
摘要
Inflammation caused by traumatic, ischemic, infectious, autoimmune or toxic injury may further trigger cancer and even death. Overexpression of hydrogen sulfide (H2S) in vivo has been identified as a biomarker for various types of inflammation. Identification-responsive fluorescence imaging probes have broad application prospects for in vivo diagnosis of inflammation. However, it is a challenge to design an imaging probe that concurrently responds to the target molecules to improve the sensitivity and specificity of inflammation detection. Herein, we designed and synthesized an activatable two-photon fluorescent probe to detect H2S. Fl-H2S had high selectivity, excellent photostable signals and low detection limit for recognizing H2S. In addition, Fl-H2S showed excellent two-photon fluorescence properties in cell and liver tissue visualization experiments, with a penetration depth of up to 126 μm in liver tissue. Most importantly, the unique probe Fl-H2S was the first probe to monitor H2S levels in a mouse air pouch inflammation model by fluorescence imaging technology. We expect Fl-H2S to become an effective tool for longitudinal monitoring of inflammation, diagnosis of inflammation and prediction of underlying pathogenesis of related diseases by detecting H2S.
科研通智能强力驱动
Strongly Powered by AbleSci AI