Abstract Two new saponins 3-O-β- d -glucopyranosyl (1 → 2)-β- d -mannopyranosyl sarsasapogenin, named timosaponin A IV(1) and (5β, 25S)-26-O-β- d -glucopyranosyl-furost-20(22)-en-3,26-diol-3-O-β- d -glucopyranosyl (1 → 4) glucopyranosyl (1 → 2)-β- d -galacopyranoside, named timosaponin B IV(2), were isolated by silica gel column chromatography and preparative HPLC from Anemarrhena asphodeloides Bge. Their structures were elucidated by chemical characters and spectroscopic analysis.