Effects of abiotic and biotic factors on Vibrio harveyi ATCC 14126T survival dynamics in seawater microcosms

AME Aquatic Microbial Ecology Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout the JournalEditorsSpecials AME 83:109-118 (2019) - DOI: https://doi.org/10.3354/ame01910 Effects of abiotic and biotic factors on Vibrio harveyi ATCC 14126T survival dynamics in seawater microcosms Maite Orruño1,2,*, Claudia Parada1, Elixabet Ogayar1, Vladimir R. Kaberdin1,2,3, Inés Arana1,2 1Department of Immunology, Microbiology and Parasitology, Faculty of Science and Technology, University of the Basque Country UPV/EHU, 48340 Leioa, Spain 2Research Centre for Experimental Marine Biology and Biotechnology (PIE-UPV/EHU), 48620 Plentzia, Spain 3IKERBASQUE, Basque Foundation for Science, 48013 Bilbao, Spain *Corresponding author: maite.orruno@ehu.es ABSTRACT: The ability of marine bacteria to adequately respond to environmental abiotic and biotic stressors ensures that they can thrive in diverse aquatic ecosystems. Here, we showed that interaction of Vibrio harveyi with natural microbiota can greatly reduce the number of vibrios, and this effect was dependent on temperature. In contrast, the impact of abiotic stress factors was less hostile, primarily affecting V. harveyi morphology and physiological state. Although exposure to an oligohaline environment (0.5‰) and nutrient limitation led to a rapid and irreversible loss of V. harveyi culturability, higher salinities (15-35.5‰) induced transition to the viable but nonculturable (VBNC) state and cell size reduction in a temperature-dependent manner. Namely, while low temperature (4°C) caused a marked loss of culturability resulting in a dominant VBNC population, higher temperatures (12, 20 and 30°C) affected bacterial culturability to a lesser extent, but resulted in shorter cells after prolonged incubation, especially at 20 and 30°C. In summary, our results define temperature as a key factor that influences V. harveyi physiology, morphology and persistence in aquatic systems. KEY WORDS: Vibrio harveyi · Temperature · Predation · Viable but nonculturable Full text in pdf format NextCite this article as: Orruño M, Parada C, Ogayar E, Kaberdin VR, Arana I (2019) Effects of abiotic and biotic factors on Vibrio harveyi ATCC 14126T survival dynamics in seawater microcosms. Aquat Microb Ecol 83:109-118. https://doi.org/10.3354/ame01910 Export citation Mail this link - Contents Mailing Lists - RSS Facebook - Tweet - linkedIn Cited by Published in AME Vol. 83, No. 2. Online publication date: July 11, 2019 Print ISSN: 0948-3055; Online ISSN: 1616-1564 Copyright © 2019 Inter-Research.