基因分型
基因型
丙型肝炎病毒
病毒学
生物
病毒
病毒载量
医学
基因
遗传学
作者
Yue Wang,Jin’e Lei,Wei Duan,Xiao Jiang,Lan Mu,Lingyun Hui,Wen-Xin Shi,Chen Zhou,Yanan Du
出处
期刊:Chinese Journal of Clinical Hepatology
日期:2017-10-30
卷期号:31 (5): 414-418
标识
DOI:10.3760/cma.j.issn.1003-9279.2017.05.008
摘要
Objective
To investigate hepatitis C virus(HCV)genotyping and the serum HCV-RNA concentration in patients infected with different HCV genotypes and to provide information for evaluation of disease condition and anti-viral treatment efficacy.
Methods
A total of 60 anti-HCV positive serum samples were collected before antiviral treatment. RT-PCR was performed for the 5′ non-cording region and was followed by nucleotide sequencing for HCV genotyping. Meanwhile, serum HCV-RNA concentration was detected by quantitative PCR. SPSS21.0 and Graphpad Prism 5.0 software were used for data analysis. Analysis of variance (ANOVA) was used for comparison among multi-groups and the t-test was used for comparison between two groups.
Results
The frequencies of HCV genotypes 1b, 3a, 1a and 2a were 48.3% (29/60), 23.3% (14/60), 16.7% (10/60) and 10% (6/60), respectively. And, there is one subtype 2c was detected in this study. The mean serum viral concentration with standard deviation of HCV in genotype 1a, 1b, 2a, and 3 a were 5.46±1.19, 6.22±0.78, 5.47±0.65, and 5.38±0.98 log10 (IU/ml) respectively.
Conclusions
The infection rate of HCV genotype 1 was significantly higher than that of genotype 2 and 3 (P<0.01). The statistical analysis showed the serum HCV-RNA concentration in patients with subtype 1b was significantly higher than that of the subtype group 1 a, 2 a, and 3 a (P<0.05). The study of the relationship between HCV genotypes and the serum HCV-RNA concentration may contribute to anti-viral treatment prescription for hepatitis C patients.
Key words:
Hepatitis C Virus; 5′non-cording region; HCV genotyping; Serum HCV-RNA concentration
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