Affinity Adsorption of Bilirubin on Cibacron Blue F3GA-modified Microporous Polytetrafluoroethylene Capillary

Bilirubin removal from human plasma was obtained via an affinity microporous polytetrafluoroethy- lene(MPTFE) capillary. The new adsorbent comprised grafted glycidyl methacrylate(GMA) via radiation-induced polymerization as hydrophilic coating and reactive sites; ethylenediamine(EDA) as a spacer arm; Cibacron Blue F3GA(CB F3GA) as an affinity ligand; MPTFE capillary as the supporting matrix. The average density of CB F3GA attachment to MPTFE capillaries was found to be 136.5 μmol/g. The capacity of bilirubin adsorbed on affinity MPTFE capillaries is 76.1 mg bilirubin/g polymer(at 25 °C). This new adsorbent has advantages over both membrane and traditional micro-column, and this system is stable and easy to operate. The results of blood tests suggest the CB F3GA affinity capillary has good blood compatibility. Keywords Bilirubin removal; Radiation grafting; Cibacron Blue F3GA; Polytetrafluoroethylene; Microporous capil- lary Article ID 1005-9040(2010)-02-323-08