Total content determination for the effective fraction of the alkaloids in Dicranostigma leptopodum (Maxim.) Fedde by HPLC and ultraviolet-visible spectrophotometry

For the quality control and the rational use of the effective fraction of the alkaloids (EFA) in Dicranostigma leptopodum (Maxim.) Fedde (DLF), extraction and determination methods for EFA were developed in the present study.