胚胎发生
体细胞
生物
胚胎发生
蛋白质组学
鸟枪蛋白质组学
细胞生物学
计算生物学
胚胎
再生(生物学)
遗传学
基因
作者
Angelo Schuabb Heringer,Claudete Santa‐Catarina,Vanildo Silveira
出处
期刊:Proteomics
[Wiley]
日期:2018-02-23
卷期号:18 (5-6)
被引量:40
标识
DOI:10.1002/pmic.201700265
摘要
Abstract Somatic embryogenesis is a biotechnological approach mainly used for the clonal propagation of different plants worldwide. In somatic embryogenesis, embryos arise from somatic cells under appropriate culture conditions. This plasticity in plants is a demonstration of true cellular totipotency and is the best approach among the genetic transformation protocols used for plant regeneration. Despite the importance of somatic embryogenesis, knowledge regarding the control of the somatic embryogenesis process is limited. Therefore, the elucidation of both the biochemical and molecular processes is important for understanding the mechanisms by which a single somatic cell becomes a whole plant. Modern proteomic techniques rely on an alternative method for the identification and quantification of proteins with different abundances in embryogenic cell cultures or somatic embryos and enable the identification of specific proteins related to somatic embryogenesis development. This review focuses on somatic embryogenesis studies that use gel‐free shotgun proteomic analyses to categorize proteins that could enhance our understanding of particular aspects of the somatic embryogenesis process and identify possible targets for future studies.
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