骨膜
去细胞化
细胞外基质
绒毛尿囊膜
细胞生物学
生物医学工程
胫骨
骨形态发生蛋白
组织工程
骨形态发生蛋白2
解剖
血管生成
化学
材料科学
生物
医学
体外
胚胎
内科学
生物化学
基因
作者
Jianfeng Zhang,Qi Zhang,Jiaxin Chen,Juan Ni,Zhiping Zeng,Gangliang Wang,Liyang Song,Shunwu Fan,Pengfei Chen,Xianfeng Lin
出处
期刊:ACS Biomaterials Science & Engineering
[American Chemical Society]
日期:2017-10-27
卷期号:3 (12): 3503-3514
被引量:9
标识
DOI:10.1021/acsbiomaterials.7b00548
摘要
The periosteum plays a key role in bone regeneration and an artificial bionic material is urgently required. The periostea on the tibia and skull differ with respect to the types of cells, microstructure, and components, leading to different biological functions and biomechanical properties. We aimed to prepare decellularized periosteum scaffolds derived from different origins and evaluate their angiogenic and osteogenic activities. Histological assessment of α-smooth muscle actin, bone morphogenetic protein-2, and alkaline phosphatase in tibial and calvarial periosteum tissues provided preliminary information on their differing angiogenic and osteogenic properties. We developed decellularization protocols to completely remove the periosteum cellular components and for good maintenance of the hierarchical multilayer structures and components of the extracellular matrix (ECM) with no cytotoxicity. Moreover, using a chicken egg chorioallantoic membrane assay and a nude mouse implantation model, we found that tibia-derived periosteum ECM had superior osteogenic activity and calvarium-derived ECM had good angiogenic activity. The preliminary mechanisms of differing activities were then evaluated by osteogenesis- and angiogenesis-related gene expression in human umbilical vein endothelial cell- and MC-3T3 cell-seeded ECM scaffolds. Thus, this study provides periosteum biomaterials that are derived from specific tissues and have different functional properties and structures, for use in bone regeneration.
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