Synthesis and Characterisation of Acyl Carrier Protein Bound Polyketide Analogues

β-oxoacyl thiolesters of N-acetylcysteamine (SNACs, see structure) were synthesised and shown to be substrates for the self-acylation reaction of the Type II actinorhodin polyketide synthase holo acyl carrier protein (ACP). The preparation of ACPs bearing analogues of polyketide biosynthesis intermediates is described.