亲爱的研友该休息了!由于当前在线用户较少,发布求助请尽量完整的填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!身体可是革命的本钱,早点休息,好梦!

An immunoinformatics-based designed multi-epitope candidate vaccine (mpme-VAC/STV-1) against Mycoplasma pneumoniae

表位 免疫原性 反向疫苗学 生物 病毒学 肺炎支原体 抗原 佐剂 微生物学 免疫学 医学 肺炎 内科学
作者
Thaís Cristina Vilela Rodrigues,Arun Kumar Jaiswal,Marcela Rezende Lemes,Marcos Vinícius da Silva,Helioswilton Sales‐Campos,Luiz Carlos Júnior Alcântara,Sthephane Fraga de Oliveira Tosta,Rodrigo Bentes Kato,Khalid J. Alzahrani,Debmalya Barh,Vasco Azevedo,Sandeep Tiwari,Siomar de Castro Soares
出处
期刊:Computers in Biology and Medicine [Elsevier]
卷期号:142: 105194-105194 被引量:5
标识
DOI:10.1016/j.compbiomed.2021.105194
摘要

Pneumonia is a serious global health problem that accounts for over one million deaths annually. Among the main microorganisms causing pneumonia, Mycoplasma pneumoniae is one of the most common ones for which a vaccine is immediately required. In this context, a multi-epitope vaccine against this pathogen could be the best option that can induce effective immune response avoiding any serious adverse reactions. In this study, using an immunoinformatics approach we have designed a multi-epitope vaccine (mpme-VAC/STV-1) against M. pneumoniae. Our designed mpme-VAC/STV-1 is constructed using CTL (cytotoxic T lymphocyte), HTL (Helper T lymphocyte), and B-cell epitopes. These epitopes are selected from the core proteins of 88 M. pneumoniae genomes that were previously identified through reverse vaccinology approaches. The epitopes were filtered according to their immunogenicity, population coverage, and several other criteria. Sixteen CTL/B- and thirteen HTL/B- epitopes that belong to 5 core proteins were combined together through peptide linkers to develop the mpme-VAC/STV-1. The heat-labile enterotoxin from E. coli was used as an adjuvant. The designed mpme-VAC/STV-1 is predicted to be stable, non-toxic, non-allergenic, non-host homologous, and with required antigenic and immunogenic properties. Docking and molecular dynamic simulation of mpme-VAC/STV-1 shows that it can stimulate TLR2 pathway mediated immunogenic reactions. In silico cloning of mpme-VAC/STV-1 in an expression vector also shows positive results. Finally, the mpme-VAC/STV-1 also shows promising efficacy in immune simulation tests. Therefore, our constructed mpme-VAC/STV-1 could be a safe and effective multi-epitope vaccine for immunization against pneumonia. However, it requires further experimental and clinical validations.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
平常的凡白完成签到 ,获得积分10
4秒前
dream177777完成签到 ,获得积分10
7秒前
monicaj完成签到 ,获得积分10
9秒前
cheese完成签到 ,获得积分10
16秒前
SHUTUP发布了新的文献求助10
16秒前
577kkmm完成签到 ,获得积分10
17秒前
墨与白完成签到 ,获得积分10
23秒前
唐汉发布了新的文献求助10
28秒前
活泼的匕完成签到 ,获得积分10
28秒前
50秒前
57秒前
59秒前
兰兰发布了新的文献求助10
1分钟前
1分钟前
orixero应助兰兰采纳,获得10
1分钟前
1分钟前
吕懿发布了新的文献求助10
1分钟前
研友_VZG7GZ应助577kkmm采纳,获得10
1分钟前
情怀应助吕懿采纳,获得10
1分钟前
高兴的店员完成签到,获得积分10
1分钟前
蓝色的纪念完成签到,获得积分10
1分钟前
横扫完成签到 ,获得积分10
1分钟前
damie完成签到 ,获得积分10
1分钟前
1分钟前
神凰完成签到,获得积分10
1分钟前
升升升呀应助lenon采纳,获得10
1分钟前
1分钟前
田様应助唐汉采纳,获得10
2分钟前
DrY发布了新的文献求助30
2分钟前
sino-ft完成签到,获得积分10
2分钟前
2分钟前
2分钟前
eri发布了新的文献求助10
2分钟前
2分钟前
2分钟前
Andy发布了新的文献求助10
2分钟前
liu576454693发布了新的文献求助10
2分钟前
123发布了新的文献求助10
2分钟前
liu576454693完成签到,获得积分10
2分钟前
小马甲应助123采纳,获得10
2分钟前
高分求助中
Un calendrier babylonien des travaux, des signes et des mois: Séries iqqur îpuš 1036
Sustainable Land Management: Strategies to Cope with the Marginalisation of Agriculture 1000
Corrosion and Oxygen Control 600
Heterocyclic Stilbene and Bibenzyl Derivatives in Liverworts: Distribution, Structures, Total Synthesis and Biological Activity 500
重庆市新能源汽车产业大数据招商指南(两链两图两池两库两平台两清单两报告) 400
Division and square root. Digit-recurrence algorithms and implementations 400
行動データの計算論モデリング 強化学習モデルを例として 400
热门求助领域 (近24小时)
化学 材料科学 医学 生物 有机化学 工程类 生物化学 纳米技术 物理 内科学 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 电极 光电子学 量子力学
热门帖子
关注 科研通微信公众号,转发送积分 2545278
求助须知:如何正确求助?哪些是违规求助? 2175612
关于积分的说明 5600084
捐赠科研通 1896314
什么是DOI,文献DOI怎么找? 946176
版权声明 565334
科研通“疑难数据库(出版商)”最低求助积分说明 503541