定制
纳米技术
模块化设计
桥接(联网)
生物物理学
材料科学
笼子
费斯特共振能量转移
化学
计算机科学
物理
生物
工程类
法学
操作系统
荧光
结构工程
量子力学
计算机网络
政治学
作者
Izabela Stupka,Yusuke Azuma,Artur Biela,Motonori Imamura,Simon Scheuring,Elżbieta Pyza,Olga Woźnicka,Daniel P. Maskell,Jonathan G. Heddle
出处
期刊:Science Advances
[American Association for the Advancement of Science (AAAS)]
日期:2022-01-07
卷期号:8 (1)
被引量:18
标识
DOI:10.1126/sciadv.abj9424
摘要
Engineered protein cages are promising tools that can be customized for applications in medicine and nanotechnology. A major challenge is developing a straightforward strategy for endowing cages with bespoke, inducible disassembly. Such cages would allow release of encapsulated cargoes at desired timing and location. Here, we achieve such programmable disassembly using protein cages, in which the subunits are held together by different molecular cross-linkers. This modular system enables cage disassembly to be controlled in a condition-dependent manner. Structural details of the resulting cages were determined using cryo–electron microscopy, which allowed observation of bridging cross-linkers at intended positions. Triggered disassembly was demonstrated by high-speed atomic force microscopy and subsequent cargo release using an encapsulated Förster resonance energy transfer pair whose signal depends on the quaternary structure of the cage.
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