化学
糖肽
糖苷键
N-糖酰胺酶F
聚糖
天冬酰胺
恶唑啉
另一个
生物化学
糖基化
肽
低聚糖
立体化学
糖蛋白
氨基酸
酶
催化作用
抗生素
作者
Yuankun Dao,Weidong Dong,Jun Zhang,Suwei Dong
标识
DOI:10.1080/07328303.2022.2027434
摘要
N-glycans in eukaryotic proteins are commonly attached to asparagine residues via β-N-glycosidic linkages, which are susceptible to glycosidases, such as PNGase. Here we report the preparation of α-N-glycosylated peptides based on the solid-phase peptide synthesis strategy using an α-N-GlcNAc-containing asparagine building block, and a transglycosylation reaction of oligosaccharide oxazoline promoted by endoglycosidases CCN180H. The resultant glycopeptide, bearing the complex-type α-N-sialyl undecasaccharide, exhibits resistance against PNGase F, which may be of potential use in studying the catabolism of N-glycans and glycoengineering those peptide-based therapeutics.
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