毛细管电色谱
环糊精
色谱法
化学
毛细管作用
毛细管电泳
分析物
相(物质)
手性柱色谱法
泮托拉唑
分析化学(期刊)
高效液相色谱法
有机化学
材料科学
奥美拉唑
医学
内科学
复合材料
作者
Weiyang Tang,Yao Lü,Kyung Ho Row,Sung Hyeon Baeck,Yifan Zhang,Guangyong Sun
标识
DOI:10.1016/j.microc.2022.107763
摘要
A capillary was co-immobilized with mixed β-cyclodextrin and bovine serum album by chemical covalent coupling as the chiral stationary phase for the enantioseparation of proton-pump inhibitors in capillary electrochromatography. The chromatographic parameters including buffer concentration, pH value, organic modifier, and applied voltage were optimized. The separation performance of the developed column was evaluated by enantioseparating omeprazole, lansoprazole, pantoprazole, and esomeprazole with extremely high resolutions of 5.21, 7.11, 6.39, and 4.75, respectively. Moreover, the mixed chiral stationary phase exhibited a broader range and could separate more than 10 chiral analytes compared to the single-selector column. The relative standard deviations of the day-to-day and column-to-column repeatability were less than 4%, demonstrating excellent stability of the fabricated column. An open tubular capillary immobilized with the bovine serum album and β-cyclodextrin as the chiral stationary phase is a promising prospect for separating chiral drugs in capillary electrochromatography because of the combination of the enantioselectivities of both individual selectors.
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