In Vitro Studies on Direct Regeneration of Nothapodytes Foetida using Shoot Tip and Nodal Explants

The effect of different concentrations of auxins and cytokinins either alone or in combination with additives was assessed to be useful on the direct regeneration of shoot tip and nodal explants of N.foetida. BA and IBA proved comparatively better than others. The highest direct regeneration percent (95.0, 92.0 in shoot tip and nodal explants respectively) was observed on the MS medium supplemented with BA (3.0mgL-1) + IBA (1.0mgL-1) and additives (adenine sulphate (50mgL-1) + glutamine (100mgL-1) + L-arginine (25mgL-1) + citric acid (0.0025%) + ascorbic acid (0.005%). Shoot tips as explants performed better by giving higher regeneration percentage and better growth of propagules in comparison to nodal explants in the establishment media.