Immobilization of trypsin on poly(alginic acid‐g‐glycidyl methacrylate‐co‐hydroxy ethyl methacrylate)

Abstract To synthesize a matrix for the immobilization of enzymes, alginic acid, a naturally occurring polysaccharide has been chosen as the backbone, and glycidyl methacrylate (GMA) and 2‐hydroxy ethyl methacrylate (HEMA) as monomers for graft copolymerization. HEMA was used to introduce hydrophilic character which would have been lost during the introduction of the hydrophobic monomer glycidyl methacrylate into alginic acid. Immobilization of trypsin onto this matrix takes place by covalent bonding. The temperature optimum of the immobilized enzyme was found to be 35°C and it was most stable at pH 8.