High-level expression, purification, and characterization of recombinant human tumor necrosis factor synthesized in the methylotrophic yeast Pichia pastoris

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTHigh-level expression, purification, and characterization of recombinant human tumor necrosis factor synthesized in the methylotrophic yeast Pichia pastorisK. Sreekrishna, Lynn Nelles, Rica Potenz, John Cruze, Paul Mazzaferro, Wayne Fish, Motohiro Fuke, Kathy Holden, Doris Phelps, and Cite this: Biochemistry 1989, 28, 9, 4117–4125Publication Date (Print):May 2, 1989Publication History Published online1 May 2002Published inissue 2 May 1989https://pubs.acs.org/doi/10.1021/bi00435a074https://doi.org/10.1021/bi00435a074research-articleACS PublicationsRequest reuse permissionsArticle Views1004Altmetric-Citations122LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts