Single-step separation of red blood cells, granulocytes and mononuclear leukocytes on discontinuous density gradients of Ficoll-Hypaque

Centrifugation of heparinized human blood on discontinuous gradients of Ficoll-Hypaque resulted in the simultaneous separation of mononuclear leukocytes, granulocytes, and erythrocytes. Nearly complete separation of cells was achieved with high recovery of each cell type. Leukocytes isolated by this method retained the ability to exclude Trypan Blue dye and granulocytes retained their chemotactic responsiveness.