适体
细胞
血小板源性生长因子受体
生物物理学
间充质干细胞
细胞膜
膜
荧光显微镜
细胞信号
化学
荧光
细胞生长
血小板衍生生长因子
材料科学
纳米技术
干细胞
生长因子
细胞生物学
生物
信号转导
分子生物学
生物化学
受体
物理
量子力学
作者
Weian Zhao,Sebastian Schäfer,Jonghoon Choi,Yvonne J. Yamanaka,Maria L. Lombardi,Sugata Bose,Adam Carlson,Joseph A. Phillips,Weisuong Teo,Ilia A. Droujinine,Cheryl H. Cui,Rakesh K. Jain,Jan Lammerding,J. Christopher Love,Charles P. Lin,Debanjan Sarkar,Rohit Karnik,Jeffrey M. Karp
标识
DOI:10.1038/nnano.2011.101
摘要
The ability to explore cell signalling and cell-to-cell communication is essential for understanding cell biology and developing effective therapeutics. However, it is not yet possible to monitor the interaction of cells with their environments in real time. Here, we show that a fluorescent sensor attached to a cell membrane can detect signalling molecules in the cellular environment. The sensor is an aptamer (a short length of single-stranded DNA) that binds to platelet-derived growth factor (PDGF) and contains a pair of fluorescent dyes. When bound to PDGF, the aptamer changes conformation and the dyes come closer to each other, producing a signal. The sensor, which is covalently attached to the membranes of mesenchymal stem cells, can quantitatively detect with high spatial and temporal resolution PDGF that is added in cell culture medium or secreted by neighbouring cells. The engineered stem cells retain their ability to find their way to the bone marrow and can be monitored in vivo at the single-cell level using intravital microscopy.
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