A DC discharge dusty plasma device used for study of waves in dusty plasma is introduced. A dusty plasma column is produced with about 30cm in length and about 8.4cm in diameter. The electron saturation current of Langmuir probe is obviously decreasing while the dust grains are present in the plasma. The negative charge on dust grains is directly proportional to the rotaion rate of the dispenser. And the dust grains carry up to 40% of the negative charges in the whole plasma.