Expression of mGluR1 in hippocampus of spontaneously epileptic rats

Aim To investigate the expression of mGluR1 in hippocampus of spontaneously epileptic rats(SER) by control study.Methods Total RNA was extracted from hippocampus of SER and Wistar rats,and mGluR1 mRNA expression was detected by RT-PCR;the expression of mGluR1 protein was detected by immunohistochemistry and Western blot.Results mGluR1 mRNA of SER was lower than that of Wistar rats in hippocampus(P0.05).mGluR1 protein in DG and CA3 regions of SER hippocampus by immunohistochemistry was decreased compared with that in Wistar rats(P0.05),but in CA1 region no significant differences(P0.05) were found.In positive cells,mGluR1 proteins were enhanced.By Western blot,total mGluR1 protein was down-regulated in SER hippocampus(P0.05).Conclusion The total expression of mGluR1 was down-regulated in SER hippocampus,and enhanced in single cells.