Separator is one of the key materials for lithium-ion batteries, because its characteristics influence on the interface composition of electrode, inter impedance of battery and impregnate amount of electrolyte for cell, then influencing rate, cycle and safe performance for batteries. The preparation, configuration and dis/advantages of microporous polyolefin separator, nonwoven separator and coated compound separators were reviewed. The key technologies and processes of separator preparation were discussed. The improvement and development of separator for lithium-ion batteries were proposed and prospected.