Objective To determine the contents of total saponin and monomer saponin Rb1 and Rb3 of Panax notoginseng flower bud in different habitats and growth ages in the major productive area of Wenshan,Yunnan. Methods Determine the content of total saponin and monmer saponin through colorimetry and high efficient liquid chromatography(HPLC) respectively. Results The contents of total saponin and monomer saponin in different habitats and ages vary significantly. The their contents of three-year Panax notoginseng are higher than those of two-year one. Conclusion This method can be used for quality evaluation of panax notoginseng flower bud.