Study on the Extraction Methods and Quantity Determination of Evodiamine and Rutaecarpine in Evodia Rutaecarpa(Juss.) Benth

The contents of evodiamine and rutaecarpine in Evodia Rutaecarpa which produced in Yangxin County,Hubei province were determined by HPLC.The mobile phase was water-methanol-acetonirtile(44∶20∶36);the flow rate was 1.0 mL·min-1;the wavelength of UV detector was at 290nm and the column temperature was 25℃.The standard curves of evodiamine and rutaecarpine were linear at the concentration range of 0～1.2μg·mL-1;the average recovery rates were 96.32% and 95.46% and the relative standard deviations were 3.05% and 1.78%,respectivety.It has good linearity,precision and repetition.3 extracting methods for evodiamine and rutaecarpine(water extraction,ultrasonic extraction and Soxhlet extraction) were compared,the results showed that the efficiency of ultrasonic extraction was higher,it also need shorter time and lower temperature,so it was a suitable method for extracting evodiamine and rutaecarpine from Evodia Rutaecarpa(Juss.) Benth.