Separation and preparation of Epigallocatechin Gallate(EGCG) from tea polyphenols was investigated by HPLC. The EGCG was enriched after green tea polyphenols(30%) was extracted by ethyl acetate. The purity of EGCG was 60%. EGCG was Separated and purified by HPLC. The high purification solution of EGCG was collected .The solution was evaporated and freeze-dried under vacuum. Finally, the product was abtained and its purity was more than 99%.