To observe the influence of Laminarina Japonica Polysaccharides (LJPS) on intestinal immunity SIgA and intestineal flora in mice. The mice were fed LJPS at doses of 250, 500, 1000 μg/g. By ELISA kit, The effect of LJPS on intestinal immunity SIgA activity were evaluated in normal mice. By microbial cultivation, the number of intestinal flora were observed. LJPS 500, 1000 μg/g enhanced secretion of SIgA and the number of Lactobacillus in mice, and LJPS 1000 μg/g enhanced the number of Bifidobacterium. It showed that LJPS increased secretion of SIgA and regulated intestinal flora in mice.