This paper mainly deals with the study on the tissue culture of Ajania pallsiana which took the adventitious shoots as the explant.The result showed that the optimal media for the initiation of the explant was MS+6-BA 2.5 mg/L+NAA 0.1 mg/L;and that for the proliferation of the bud clusters was MS+BA 1.5 mg/L+NAA 0.3 mg/L.The best sucrose concretration was 2.5%.