Relationship of rs13266634 polymorphism in SLC30A8[solute carrier family 30,member 8)gene with type 2 diabetes in Chinese Han population

Objective To study the single nucleotide polymorphism of rs13266634(the genotypes and the frequency)in SLC30A8 gene and its association with type 2 diabetes,so as to probe into the involvement of SLC30A8 in the etiology of type 2 diabetes(T2DM).Methods The rs13266634 polymorphism of SLC30A8 gene was genotyped in 765 Han nationalities living around Chongqing,including 454 patients with type 2 diabe- tes(T2DM group)and 311 normal controls(NC group),by polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP)method.Waist circumference,body mass index(BMI),plasma glucose,se- rum insulin and lipid profile,high-sensitivity C-reactive protein(hsCRP)and non-esterified fatty acid(NEFA) were all measured.Homa-IR and Homa-βwere analyzed to estimate the insulin resistance andβ-cell function. Results①In T2DM group,the frequencies of C allele(X~2=8.29,P=0.004)and CC genotype of rs13266634 were significantly higher than those in the NC group(X~2=9.38,P=0.009);the TT genotype was significantly lower than that of the NC group(P0.05).The C-allele of rs13266634 significantly increased T2DM risk with an allelic odd ratio(OR)of 1.36(OR=1.36,95%CI=1.11-1.67).Compared with non-carriers,heterozy- gous and homozygous carriers of C allele were liable to have type 2 diabetes with OR=1.55(95% CI=1.07- 2.25,X~2=5.42,P=0.02)and 1.75(95%CI=1.17-2.61,X~2=7.38,P=0.006),respectively.②The genotypes of CT and CC were associated with decreased insulin secretion but not with increased insulin resistance.Conclusion These results indicate that C allele polymorphism of rs132666634 site in SLC30A8 gene might be a risk factor for T2DM,suggesting that SLC30A8 might be a susceptible gene of T2DM in the Chinese Han population.