Extraction,isolation and determination of the polysacchrides from dioscorea opposita

OBJECTIVE To isolate and determine the contents of polysaccharides from dioscorea opposite.METHODSCrude polysaccharides were obtained with water extraction,alcohol and CTAB precipitation successively,gelcolumn chromatographyon SephadexG-100 and HPLC analysis were used to purify the polysaccharides and determine their molecular weights,and to determine the contents of polysaccharides.RESULTSTwo homogenous polysaccharides were isolated from the root of dioscorea opposita and the molecular weights of S1 and S2 were 62 000 and 7 300 Dal respectively;The contents of polysaccharides were 16.42%.CONCLUSIONThe polysaccharides can be extracted and isolated by the methods of the experiment,compared the determine method by HPLC with other methods,the results of the contents of polysaccharides were accurate,and the methods can be used to determine the contents of polysaccharides.