Rumen liquid got from four XuHuai White goats fitted with the ruminal cannulae was fermented to determine the effects of four different substrates(Roughage/Concentrate: 10∶0、 8∶2、 6∶4、(4∶6)) on the pH,volatile fatty acids(VFA) concentration in culture fluid in vitro.The results showed that with the increase of R/C,the fermentation in vitro increased.When the R/C was 8∶2 and 6∶4,the fermentation showed the highest level,and the control group was always at the lowest level.