适体
卡那霉素
检出限
显色的
化学
过氧化物酶
比色法
组合化学
色谱法
纳米技术
抗生素
材料科学
生物化学
酶
生物
分子生物学
作者
Yue Tang,Yang Hu,Pei Zhou,Chunxiao Wang,Han Tao,Yuangen Wu
标识
DOI:10.1021/acs.jafc.1c00925
摘要
Although the colorimetric methods can easily meet the demands of point-of-care and ease-of-use for antibiotic detection, they still face many challenges in the accuracy and stability of assay. Herein, a facile and stable colorimetric aptasensor is first developed for kanamycin residue detection based on the aptamer-enhanced peroxidase-mimicking activity of layered WS2 nanosheets. The investigation confirmed that aptamer sequences can improve the affinity of nanosheets to the chromogenic substrate 3,3'',5,5''-tetramethylbenzidine, resulting in a significant increase of the peroxidase-mimicking activity. Under the optimal conditions, the limit of detection of the proposed colorimetric aptasensor for kanamycin was determined to be as low as 0.6 μM, and such an aptasensor displays excellent selectivity against other competitive antibiotics. Moreover, further studies have verified the applicability of the established colorimetric aptasensor in several actual samples, indicating that the aptasensor may have bright application prospects for kanamycin detection in livestock husbandry and agriculture samples.
科研通智能强力驱动
Strongly Powered by AbleSci AI