Capture and metabolomic analysis of the human endometrial epithelial organoid secretome

Significance The ability to robustly isolate intraorganoid fluid in a high-throughput capacity, using human endometrial epithelia as a model, circumvents several constraints typically associated with obtaining biological fluids, both in vivo and in vitro. This approach also allows for direct tandem genomics, transcriptomics, proteomics, and functional metabolomics. In addition to enhancing our understanding of uterine fluid composition regulation, this innovation is directly and readily transferrable to wider fields of cell and organoid biology, with implications for personalized, or precision, medicine approach development.