双歧杆菌
单核细胞增生李斯特菌
抗氧化剂
过氧化氢异丙苯
硫氧还蛋白
微生物学
硫氧还蛋白还原酶
谷胱甘肽还原酶
超氧化物歧化酶
化学
生物化学
谷胱甘肽
生物
氧化应激
谷胱甘肽过氧化物酶
细菌
酶
双歧杆菌
乳酸菌
遗传学
催化作用
发酵
作者
Xiaomin Yu,Xiaoli Wu,Nagendra P. Shah,Feng Xu
标识
DOI:10.1016/j.lwt.2020.109209
摘要
In this study, we found that the antioxidant activity of Bifidobacterium bifidum WBBI03 and Listeria monocytogenes CMCC 54001 was increased after co-incubation. Therefore, the molecular mechanism of enhancing antioxidant activity was explored through isobaric tags for relative and absolute quantitation (iTRAQ). Analysis of differentially expressed proteins revealed increased expression of a series of antioxidant-related proteins, including glutaredoxin and thioredoxin in B. bifidum WBBI03, and universal stress protein UspA and aldo/keto reductase in L. monocytogenes CMCC 54001. Based on previous studies, certain antioxidant genes were selected for fluorescence quantitative PCR analysis to find out transcriptionally up-regulated genes. It was also detected that superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX) enzyme activities increased after interaction of the two strains. These results may partially explain the improved survival of B. bifidum WBBI03 and L. monocytogenes CMCC 54001 after interaction in strong oxidizing environments containing hydrogen peroxide or cumene hydroperoxide (CHP). It is speculated that L. monocytogenes (a representative opportunistic pathogenic bacterium) may help B. bifidum (a representative probiotic bacterium) to resist damages caused by oxygen species in the gastrointestinal tract, which may part of the biological significance of its presence in the intestinal microbial ecosystem.
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