可重用性
纳米材料
样品制备
萃取(化学)
纳米技术
蛋白质纯化
色谱法
化学
计算机科学
材料科学
软件
程序设计语言
作者
Estefanía González-García,María Luisa Marina,María Concepción García
标识
DOI:10.1080/15422119.2019.1581216
摘要
Protein sample preparation is the most critical step in protein analysis of complex samples and is constituted by tedious, time-consuming, and difficult-to-automate steps that usually involve the use of high volumes of solvents. In recent years, novel extraction or digestion nanomaterials (NMs) have been developed aiming to overcome these drawbacks. In this review, we have grouped the recent works related to the development of new NMs and their applications to the extraction, enrichment/purification, and digestion of proteins. This paper evaluates the role of different kinds of NMs in each step of protein sample preparation focusing on the type of established interaction between the protein and the nanomaterial, their sensitivity and selectivity, their adsorption capacity, and the advantages that they bring in relation to time, efficiency, or reusability.
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