纳米团簇
荧光
检出限
双金属片
化学
RGB颜色模型
半胱氨酸
铜
肉眼
光化学
金属
核化学
分析化学(期刊)
材料科学
光学
色谱法
计算机科学
有机化学
酶
物理
操作系统
作者
Azad H. Alshatteri,Khalid M. Omer
标识
DOI:10.1016/j.microc.2023.108385
摘要
Ratiometric-based probes improve detection precision and accuracy by minimizing the influence of various experimental conditions. Here, we present a unique dual-emission fluorescence probe constructed by a dual-nanocluster. This sensing platform is composed of copper nanoclusters (CuNCs) as a reference signal and silver nanoclusters (AgNCs) as a response signal. Uracil was used as a capping agent to improve the stability of AgNCs by forming a complex with silver metal. Ratiometrically, the composite of copper/silver bimetallic nanoclusters (Cu@Ag NCs) was used to measure the amount of cysteine as a typical biothiol. The Cu@Ag NCs showed a reddish fluorescence that originated from AgNCs and CuNCs when excited by a UV lamp. Under UV illumination, the addition of cysteine caused a noticeable fluorescence color shift from red to blue. Due to the color tonality, a smartphone and the Color Grab app were used to analyze red, green, and blue (RGB) values from the images of the dual-nanocluster solutions before and after the addition of cysteine. A satisfactory linear correlation between the ratio of red to blue values as a function of cysteine concentration was established for the quantitative measurement of cysteine from 20 to 180 μM with a limit of detection of 7.0 μM. The proposed probe was applied to assay cysteine in human serum with satisfactory accuracy and precision.
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