Nanozymes refer to the nanomaterials with enzyme-like characteristics. Currently, the introduction of light is considered as an effective means to regulate the catalytic activity of nanozymes. In this chapter, we focus on the progress of photoresponsive nanozymes. The interconnection between the photoactivity and enzyme-like activity of nanozymes is discussed. In addition, the types of photoresponsive nanozymes based on various nanostructures, including metal-based, metal compound-based, carbon-based, and MOF-based, are presented. Finally, the biomedical applications of photoresponsive nanozymes are highlighted.