Electro-switchable addressing system for achieving repetitive random data access

Abstract Due to its high physical density and excellent durability, DNA data storage has become an attractive storage solution. Efficient and fast random access to the required storage data is a key element of any data storage system. Here, we present a method of using electrically driven DNA conformation to promote or inhibit T7 promoter transcription in order to random access DNA information. Electrical actuation provides physical addresses for accessing specific information without the need to design complex sequence address sets to expand the space of encoded sequences and increase theoretical storage density and capacity. At the same time, the T7 promoter transcribes DNA information in situ without damaging DNA, achieving multiple information reads with one write. In addition, the random access to the electrode array chip reveals the possibility of future compatibility with DNA synthesis, laying the foundation for future miniaturized storage integration.