化学
荧光
细胞器
脂滴
生物物理学
共焦
聚集诱导发射
细胞内
分子探针
活体细胞成像
荧光寿命成像显微镜
分子成像
共焦显微镜
纳米技术
生物化学
细胞
细胞生物学
物理
材料科学
生物
DNA
几何学
数学
生物技术
量子力学
体内
作者
Zhiyu Wang,Yi Deng,Jiayi Ge,Feiyang Ding,Hailing Du,Xuebin Shen,Cuifeng Zhang,Xiaoxia Gu,Lei Hu,Hui Wang
标识
DOI:10.1016/j.tetlet.2024.155070
摘要
As essential subcellular organelles, lipid droplets (LDs) are involved in various the pathophysiological processes of metabolic diseases, including fatty liver and diabetes. Therefore, detecting LDs is conducive for studying the pathogeny and the early diagnosis of associated diseases. In this work, a series of fluorescent probes L1-L3 based on triphenylamine derivatives were designed and synthesized for tracking LDs in living cells. Due to the different terminal moieties of the three probes, L1 and L3 are aggregation-induced emission (AIE)-active, while L2 is a dual-state emission (DSE) molecule. In addition, confocal cell imaging experiments showed that probes L1-L3 specifically target intracellular LDs sites without the post washing. Importantly, probe L3 allows real-time monitoring of the dynamic processes and changes in the number of LDs. In summary, probe L3 is an ideal probe for studying LDs-related pathophysiological processes, and this work provides more ideas for the future design and synthesis of high-performance fluorescent probes for targeting LDs.
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