Metabolomics Analysis of Urine and 16S rRNA Sequencing of Gut Microbiota in Rats With Blood Stasis Syndrome After Intervention With Danggui‐Guizhi Decoction and Dispensing Granules  

ABSTRACT Introduction Blood stasis syndrome (BSS) is one of the common syndromes in traditional Chinese medicine. The Chinese medicine compound composed of “Danggui‐Guizhi (DG‐GZ)”as the core has the effect of reducing blood stasis and promoting blood circulation. However, the difference of efficacy of DG‐GZ traditional decoction and dispensing granules in reducing blood stasis are still unclear. Objective Investigation of the difference of decoction and dispensing granules of Danggui (DG) and Guizhi (GZ) in blood stasis syndrome (BSS) in an animal study (Six groups, n = 6). Methodology All rats were randomly divided into six groups: control, model, Danggui—Guizhi dispensed granules ( DGK–GZK), Danggui—Guizhi decoction (DG–GZ), Danggui granules—Guizhi decoction (DGK–GZ), and Danggui decoction —Guizhi granules (DG‐GZK) groups ( n = 6). The blood stasis model was constructed by exposing rats to ice‐cold water. Whole blood viscosity (WBV) was used to compare the effects of DG–GZ (4:3 w / w ) decoction and dispensing granules in improving BSS. Metabolomics analysis of urine and 16S rRNA gene sequencing were used to investigate metabolic analysis and gut microbiota changes in rats with BSS. Results The results of WBV showed that DG‐GZ and its granules exhibited efficacy against blood stasis, and the effect of DGK‐GZK in relieving blood stasis was greater than that of other administration groups. Urine metabolomics demonstrated that DGK–GZK could reverse the abnormal levels of butyrate, citrate, taurine, creatinine, glucose, and other metabolites in BSS rats. DGK–GZK could promote the enrichment of the g__Prevotellaceae _NK3B31_group, g__Ligilactobacillus , and g__Roseburia . Conclusions The effect of DGK‐GZK intervention in BSS was stronger than that of other compound groups. This study also provides basic experimental data for exploring the difference in efficacy between traditional decoction and dispensing granules.