Extraction and Characterization of Chitosan from Eco-Green <i>Hermetia Illucens</i> for Application in Dentistry

Chitosan can be extracted from the hermetia illucens chitin. It has excellent biocompatibility, non-toxic, bioresorbable, anti-inflammatory and wound healing properties. It can be formed into a gel, granules, sheet/film, porous structure, or scaffold in dental medications. Hermetia illucens cocoons were collected and extracted from the by-product of organic waste management in Bandung City. The samples were dried in the oven at 105°C for 48h and grounded in the powder grinder and sieved. Demineralization proces were done by immersed samples in 1 M HCl for 6h. The powder then deproteinized using 1 M NaOH solution for 8h at 80 o C, filtrated, and washed with distilled water. Then, the samples were immersed in 1% KMnO4 solution for 1h, and cleaned the residue with an oxalic acid solution. Samples obtained were dried in the oven at 60 o C for 48h before deacetylated with deionized water. Characterization were done by Fourier Transform Infrared spectroscopy (FTIR), and Scanning Electron Microscope (SEM). IR spectra showed disctinctive OH bond at 3253.62 cm -1 and amide I band at 1616.98 cm -1 . Degree of deacylated were calculated using infrared spectra data and resulting 77.372% indicating good character of chitosan. The result SEM were inducated typical pattern of chitosan. Further study is to fabricate the medical-grade chitosan for dental material. The chitosan from the hermetia illucens is a promising natural biopolymer for dental applications and organic waste management solutions.