化学
扫描电镜
光毒性
荧光
斯托克斯位移
吸收(声学)
菁
受激发射
生物物理学
光化学
光学
激光器
生物化学
物理
体外
生物
作者
Xiaojie Ren,Chao Wang,Xia Wu,Mengtao Rong,Rong Huang,Liang Qi,Tianruo Shen,Hongyan Sun,Rui-Long Zhang,Zhongping Zhang,Xiaogang Liu,Xiangzhi Song,James W. Foley
摘要
Superior photostability, minimal phototoxicity, red-shifted absorption/emission wavelengths, high brightness, and an enlarged Stokes shift are essential characteristics of top-tier organic fluorophores, particularly for long-lasting super-resolution imaging in live cells (e.g., via stimulated emission depletion (STED) nanoscopy). However, few existing fluorophores possess all of these properties. In this study, we demonstrate a general approach for simultaneously enhancing these parameters through the introduction of 9,9-dimethyl-9,10-dihydroacridine (DMA) as an electron-donating auxochrome. DMA not only induces red shifts in emission wavelengths but also suppresses photooxidative reactions and prevents the formation of triplet states in DMA-based fluorophores, greatly improving photostability and remarkably minimizing phototoxicity. Moreover, the DMA group enhances the fluorophores' brightness and enlarges the Stokes shift. Importantly, the "universal" benefits of attaching the DMA auxochrome have been exemplified in various fluorophores including rhodamines, difluoride-boron complexes, and coumarin derivatives. The resulting fluorophores successfully enabled the STED imaging of organelles and HaloTag-labeled membrane proteins.
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