Determination of chromium species in water using diphenylcarbazide with a sequential spectrophotometric discrete robotic analyser

There is an increasing need for fast and reliable analytical methods for the determination of chemical forms of elements in environmental samples. The interest in chromium is driven by the fact that its toxicity depends on its oxidation state. Although chromium (III) is essential for mammals to maintain their metabolism of proteins, fats, and carbohydrates, chromium (VI) is toxic to humans. For chromium speciation, several costly analytical methods coupling separation methods with atomic absorption and emission spectroscopy have been developed. This article presents the online robotic discrete analyser procedure with the 1,5 diphenylcarbazide (DPC) method for the speciation of Cr (III) and Cr (VI). Cr (III) was determined by difference since it does not interfere with the reaction of Cr (VI)-DPC. Chromium (VI) and total chromium were characterised sequentially (after online oxidation of Cr (III) by Cerium (Ce (IV)). The calibration graphs were linear under experimental conditions up to 1 mg/L Cr (VI) and 2 mg/L total Cr with correlation coefficient R